TheraKan Protocols

TM

TM

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Establishing 3D cultures in TheraKan devices
Notes:  Procedure for assembling devices and establishing 3D cultures in devices. Emphasis on how to use devices
Cytokine release assay
Notes: May also apply to measurement of other soluble proteins (growth factors, angiogenic factors, proteases etc)
Drug efficacy/toxicity assay- option 1. Notes: This assay is useful for assessing efficacy and/or toxicity of drug compounds to immune response and 3D cultures.
FITC-dextran uptake assay. Notes: This assay is useful for characterizing uptake of different sized molecules to 3D matrices. May also be used to assess other  dextrans or chemical agents labeled with different fluorochromes
Protocol for 3D collagen cultures in TheraKan devices
Notes:  Procedure for establishing 3D collagen cultures in devices. Emphasis on preparing collagen matrices.
3D Immune response assay. Notes: basic procedure on how to measure recruitment of immune cells (PBMCs or cell lines) to TheraKan devices
Drug efficacy/toxicity assay- option 2.
Notes: This assay is useful for assessing efficacy and/or toxicity of drug compounds to immune response and 3D cultures.
FITC-dextran release assay. Notes:This assay is useful for characterizing how 3D cultures affect release of different sized molecules. May also be used to assess other  dextrans or chemical agents labeled with different fluorochromes