The TheraKan system can be used to assess cellular immune recruitment and activation to 3D microenvironments over time, providing a more realistic window into cellular immune responses.
Examples of how immune response assays in the TheraKan can be used to assist clients:
Examine molecular pathways involved in cellular immune response
Examine interactions between immune cells and other cell types in tailored 3D microenvironments
Examine drug interactions with immune cell types and other cell types in tailored 3D microenvironments
Fennik Life Sciences works with clients to tailor TheraKan studies to their research needs, timeframe and budget.
What kind of data can clients expect?
The TheraKan assay can track the number of immune cells recruited to 3D cultures through live or fixed imaging and provide data on immune biomarker expression through a variety of approaches including microscopy, flow cytometry and ELISA (Figure 1). Clients receive a written report on their studies with their data, and time with staff to discuss their data through flexible scheduling.
Types of Assays offered in the TheraKan
Monocyte/Macrophage infiltration assay
Obtain imaging or flow cytometry data on recruitment of monocyte/ macrophages to 3D cultures using primary or immortalized cell lines
PBMC infiltration assay
Obtain imaging or flow cytometry data on recruitment of PBMCs to 3D cultures