A 2D & 3D Culture System Platform

TheraKan Demo Video

Tissues are dynamic environments, in which cells and molecules are constantly moving in and out.


The TheraKan™ is designed to mimic these features of living tissues and cells. The device fits into conventional plates to create multiple environments for cell culture. Our patented chamber design enables the establishment of 3D cultures with flow-through accessibility to cells and molecules from the outside.

What can be performed with the TheraKan™?

Analyze drug efficacy, toxicity, stability or visualize changes in cell behavior in or out of the chamber over time. The TheraKan™ complements existing animal models to assess mechanisms of action, or it may be used to screen ineffective drug compounds from further testing in animal models.

Other assays include:

Immune infiltration/migration by microscopy, specifically macrophage infiltration/migration

Cytokine measurement by ELISA

Fix and embed 3D cultures and analyze other biomarkers like cell proliferation or apoptosis markers


The TheraKan™ is comprised of a patented 2 chamber nested design with an easy swiveling mechanism and an opening at the bottom to control for flow of fluid, molecules and cells. To establish 3D cell cultures, close the TheraKan™ shut, and culture cells in 3D protein matrices to your specifications. When ready, swivel the device open, and test for drug interactions with tissue barriers, target cells and/or secondary interacting cell types such as white blood cells. 

Our chamber design provides a 3D and realistic dynamic environment for cells to interact mimicking what happens in the human body

MDA-MB-231 cells were cultured in 3D collagen in the TheraKan™, transfected with Ca-TAT/siRNA complexes, and analyzed for macrophage infiltration into the device.

Macrophage infiltration was quantified by Image J;

arbitrary units shown. Statistical analysis was performed by One way ANOVA followed by Bonferonni post-hoc analysis.

Statistical significance was determined by p-value less than 0.05. 

*p<0.05, **p<0.01, ***p<0.0001, n.s=not sigificant. Mean+SEM is shown.

The TheraKan™ provides stable and long-term gradients up to 48 hours, 40x longer over a standard 2D cell culture insert

Gradient Stability of 2D Insert vs TheraKan

200 ul of 500 mM crystal violet was added to 8 micron 2D cell culture inserts in a 24 well plate containing 400 ul PBS. Solutions at the bottom and topside were removed and transferred to a new plate, and photographed. Representative images and end point concentrations shown. Loss of gradient stability shown immediately.


Crystal violet delivered through the TheraKan™ photographed over time, and measured over for changes in concentration over time (color intensities) and distance from the TheraKan opening. Long-term and stable gradients for up to 48 hours.

The TheraKan™ provides better pre-clinical predictor models and lowers attrition rates over time

Prostate cancer PC-3M cells were seeded in the device with the 3-D collagen gel procedure or in 2-D 6-well plate overnight. Cells were treated with the solvent control DMSO or Alteronol at 10 mM for 24 h. All cell nuclear was stained with Hoechst33342 (blue) and dying cells were evaluated by PI staining (red). There were obvious differences of the cell death rate in response to Alteronol treatment as seen in these two different culture system,  higher death rate was found in 2-D (95%) culture than 3-D (62%) culture.

Why Choose the TheraKan™?

                          CHAMBER               2D/3D                MODELS
Easy set-up & walk away
Low cost per test (>$5)
Analysis of 3D cell cultures
Measurement of human blood cell recruitment
Model blood vessels
Analysis of tissue barriers
Fits in varying cell culture platforms
Portable: more control assays

Fennik Life Sciences, LLC

Bioscience and Business Technology Center

2002 West 39th Avenue 

Kansas City, KS 66103

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