Frequently Asked Questions

General

How do I purchase?


Please visit our SHOP page under the TheraKan tab or simple click here:

You can also email us here: orders@fenniklifesciences.com if you’d like to place an order.




How do I reset my password?


You can retrieve your password by clicking on the Forgot your password? link at the bottom of the login screen. An email will be sent to you. When clicking the link, you will be directed to a site where you can enter a new password.




Can I reset my username?


Unfortunately at this time, the web platform that we use does not include this option to reset your username.




What is your refund and exchange policy?


Please contact us at orders@fenniklifesciences.com if you have any issues with your order. If your package came damaged, please contact us so we can send you a replacement order at no additional fee. If you are unhappy with your order, you may return your un-opened devices with a restocking fee, at 25% of the cost. Contact us and we will send you a pre-paid shipping label and instructions.




Is it safe to use and store my credit card number info?


Our store is secured by HTTPS / TLS encryption and it is safe for you to purchase from our store.

TLS (Transport Layer Security) is the standard security technology for establishing an encrypted link between a web server and a browser. This link ensures that all data passed between the web server and browsers remain private and secure.




What is your shipping policy?


Please Note: Only 1 order maximum allowed per address for the Trial pack. Apologies, we can only ship to business/commerical addresses (no residential addresses).





Technical

How do I use the device?


Watch the video to learn how to use the devices: https://www.fenniklifesciences.com/therakan

Or download a printable protocol: https://www.fenniklifesciences.com/therakan




What cells can I use with these devices?


You can potentially culture any cell type with these devices. Rodent and human cell types have been used. Cell culture in devices depend on factors including matrix and media. You will need to determine the appropriate matrix and media conditions for your cell type.




How do I collect data from devices?


You can visualize immune cells infiltrated to devices through a variety of approaches.

By microscopy, we recommend an inverted microscopy with fluorescence capability to detect immune cells that have been fluorescently labeled by anti-body staining, fluorochrome labeling or tagged with fluorescent protein. Please refer to publications for examples:

https://www.oncotarget.com/article/9885/

https://www.nature.com/articles/s41598-018-29683-1

https://www.nature.com/articles/s41388-019-1141-7?proof=true19

For antibody staining, reliable results may be obtained through fixation and sectioning. Please refer to our protocols section.

You can also harvest cells from 3D cell cultures into single cell suspensions and analyze protein biomarker expression by flow cytometry or immunoblot analysis. You can also analyze RNA expression by RT-PCR. Procedures for harvesting cells from 3D cultures will depend on the matrix used. For digestion of collagen cultures, refer to our protocols section.

For digestion of Matrigel cultures, Dispase solution (cat no 35425) or recovery solution (cat no. 35423) from Corning is recommended.

You can also collect media from devices to measure protein expression by ELISA. Please refer to our protocols section.





Trouble Shooting

Problems with establishing 3D cultures in TheraKan™ devices





Problems with cell viability in TheraKan™ devices in an opened system.





Problems with detecting immune cells in TheraKan™ devices.






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