Drug uptake to 3D cultures

1.Establish 3D cultures in devices

2.Place in test chamber, open device

3.Pipet drug to test chamber

4.Assess drug uptake in devices by biochemical means

Example

FITC dextran uptake was assessed to collagen gels in the TheraKanTM device after 12 hours. Devices were imaged using an inverted fluorescence microscope.