cytokine release.tif

Cytokine release from 3D  cultures

1.Establish 3D cell cultures in devices

2.Place in test chamber; open devices

3.Assess cytokine release into test chamber by ELISA or other biochemical methods

Example

IL6 secretion.tif

IL-6 concentration (pg/ml)

MDA-MB-231 breast cancer cells were cultured in collagen in duplicate TheraKan     devices for 24 hours. Media was sampled in the test chamber for IL-6 secretion by ELISA.

TM