The TheraKan™ provides more stable and longer term gradients over a standard cell culture membrane insert
Gradient Stability in the cell culture membrane insert
200 ul of 500 mM crystal violet was added to 8 micron 2D cell culture inserts in a 24 well plate containing 400 ul PBS. Solutions at the bottom and topside were removed and transferred to a new plate, and photographed. Representative images and end point concentrations shown. Loss of gradient stability shown immediately.
Gradient Stability in the TheraKan™ device
Crystal violet was delivered through the TheraKan™ photographed over time, and measured over for changes in concentration over time (color intensities) and distance from the TheraKan opening. Long-term and stable gradients for up to 24 hours.