Frequently Asked Questions
How do I use the TheraKan device?
To access learn how to use devices in experiments, go to:
What cells can I use with TheraKan devices?
You can potentially culture any cell type with these devices. Devices have been tested with rodent and human cell types. 3D cultures in devices depend on factors including matrix and media. You may need to determine the appropriate matrix and media conditions for your cell type.
For guidance, you may access:
How do I collect data from TheraKan devices?
You can measure the number of immune cells infiltrated to devices from the test chamber, using live cell imaging approaches, or immunostaining and detection of cells by microscopy or flow cytometry.
You can examine biomarker expression in 3D cultures established in the TheraKan device using microscopy or flow cytometry techniques.
You can detect expression of soluble factors including cytokine levels by ELISA.
For further guidance, please go to protocols section at https://www.fenniklifesciences.com/protocols or publications at https://www.fenniklifesciences.com/protocols
Other potential data may include detection of gene expression by qPCR or protein expression by immunoblotting of 3D cultures. As we develop new protocols and methods for the TheraKan device, our protocols and publication sections will be updated. So please check back from time to time.
Do I need to use the lubricant provided with the device ?
The chamber devices are designed to ensure a tight fit to prevent leakage, so that 3D cultures can be established consistently, while allowing the chambers to swivel back and forth. The lubricant makes it easier to assemble the devices and swivel the chambers to open and close the system. The lubricant is inert and non-toxic, and has not been shown to affect cell systems established in the device.